Effects of iron availability on male Drosophila fertility. Iron chelation by EDTA decreased male fertility of a standard Drosophila strain, w1118 (P< 0.0001) over a 10-day mating period (A). Iron supplementation with ferric ammonium citrate (FAC) had no effect on male fly fertility (P> 0.05) (B). Data are presented as means ± SEM per day and analyzed by Two-way ANOVA. Sample sizes for each treatment were as follows: n=18, 15, 19 for 0μM, 250uM and 500μM EDTA, respectively, and n=12, 15, 16 for 0mM, 5mM and 10mM FAC, respectively.