Figure 4.

Lifespan and fertility of mitoferrin and transferrin mutants compared to a standard laboratory fly strain, w¹¹¹⁸. Hypomorph mutants, dmfrn^BG00456 and tsf1^f05108 exhibit longer lifespans (P<0.0001) than w¹¹¹⁸ flies (A). Hypomorph mutants displayed reduced male fly fertility compared to w¹¹¹⁸ (B). Lifespans were analyzed by Mantel-Cox log-rank test. For each fly strain, lifespan sample sizes, mean lifespan (means ± SEM), maximum lifespan (days) and percent increase compared to w¹¹¹⁸ were as follows: dmfrn^BG00456 n=117, 51 ± 1.7 days, 88 days, 42%; tsf1^f05108 n=124, 43 ± 1.4 days, 76 days, 19%; w¹¹¹⁸ n=124, 36 ± 1.3 days, 60 days. Fertility experiments were analyzed by student’s t-test and data presented as means ± SEM, ***P≤0.0001, n=20 mating pairs.