Table 1.
Information for primers used. GAPDH and HMBS were reference genes.
| Gene | Accession Number | Direction | Sequence | Ta (°C) | Product (bp) |
|---|---|---|---|---|---|
| TH | XM_002198931.2 | Forward | GCCATGCTGAACCTCTTCTT | 60 | 298 |
| Reverse | GATGGTGGCACTTGTCCAAT | ||||
| D1 | NM_001243833.1 | Forward | ACGAGAGGAAAATGACCCCC | 58 | 112 |
| Reverse | GTTGTAGCCTTGTGCCAGTT | ||||
| D2 | XM_002191611.2 | Forward | TACCAGTCCCCCTGAGAAAG | 58 | 96 |
| Reverse | GTAGAGTTGTTGCCCCGATT | ||||
| NT | NM_001245684.1 | Forward | TGCTCAGATTCAGAAGAGGA | 58 | 169 |
| Reverse | CTACCTCTACTGTTTCCCCC | ||||
| NTR1 | NM_001245982.1 | Forward | ATTGCCTTCGTGGTCTGTTG | 58 | 295 |
| Reverse | AAGTGTGGTTGCTGGAGATG | ||||
| GAPDH | NM_204305.1 | Forward | AGCAATGCTTCCTGCACTAC | 57.5 | 121 |
| Reverse | CTGTCTTCTGTGTGGCTGTG | ||||
| HMBS | XM_002187761.1 | Forward | ACCTGCCAACTTCTCTTCCT | 58 | 140 |
| Reverse | GGTTCCAATCACACTCTTTTCA |