Table 1.
Gene-specific primers for qRT-PCR analysis
| Gene | Primer sequence | Amplicon size (nt) | GeneBank accession # | |
|---|---|---|---|---|
| MCP-1 | Forward | TAGCATCCACGTGCTGTCTC | 94 | NM_031530.1 |
| Reverse | CAGCCGACTCATTGGGATCA | |||
| NLRP3 | Forward | TTCCCAGACCCTCATGTTGC | 306 | NM_001191642.1 |
| Reverse | CAGGGCATTGTCACTGAGGT | |||
| CASP1 | Forward | GCCGTGGAGAGAAACAAGGA | 319 | NM_012762.2 |
| Reverse | ACCCTTTCAGTGGTTGGCAT | |||
| IL18 | Forward | GACCGAACAGCCAACGAATC | 84 | NM_019165.1 |
| Reverse | TAGGGTCACAGCCAGTCCTC | |||
| IL1β | Forward | GAGTCTGCACAGTTCCCCAA | 88 | NM_031512.2 |
| Reverse | TGTCCCGACCATTGCTGTTT | |||
| IL6 | Forward | GCAAGAGACTTCCAGCCAGT | 143 | NM_012589.2 |
| Reverse | CCTCCGACTTGTGAAGTGGT | |||
| ASC | Forward | GGACAGTACCAGGCAGTTCG | 140 | NM_172322.1 |
| Reverse | GTCACCAAGTAGGGCTGTGT | |||
| GAPDH | Forward | TCAAGAAGGTGGTGAAGCAG | 111 | NG_028301.2 |
| Reverse | AGGTGGAAGAATGGGAGTTG |