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. Author manuscript; available in PMC: 2018 Jan 1.
Published in final edited form as: Biomaterials. 2016 Oct 28;113:118–132. doi: 10.1016/j.biomaterials.2016.10.046

Figure 4.

Figure 4

Expression of distal lung epithelial lineage markers in airway organoids. H&E staining of airway organoid section (A). Co-staining of E-cadherin (red) and Vimentin (green) reveals diverse architecture of epithelial and mesenchymal compartments within airway organoids at day 21 in vitro with low- and high-power imaging (B–D). AECI markers AQP5 (red) and PDPN (green) were seen throughout the airway organoids with low- and high- power magnification (E, F), as well as with E-cadherin (green) in the airway organoids (G). Co-staining AQP5 (red) with CC10 (green) in the airway organoids with low- and high- power magnification (I, J). AECII marker SPC (red) co-staining with Vimentin (green) the airway organoids (H). Co-staining CC10 (red) with HOPX (green) in the airway organoids with low- and high- power magnification (K, L). Relative gene expression of SPC, AQP5, HOPX, and PDPN in airway organoids at different time points compared to NHBE 2D cell culture (M). Values in graphs represent mean ± SEM; n=3, *P < 0.05; **P < 0.01; t-test. Scale bars, 200µm (A, B), 100µm (E, G, H, I, K), 50µm (C, D, F, J, L).