Figure 4. Sumoylation of Smo at K851 is required for its optimal activity in vivo.

(A-A′) Wing imaginal discs of late third instar larvae containing smo³ clones induced by the MARCM system were immunostained to show the expression of GFP, Ci, En (blue in A) or Ptc (blue in A′). smo³ clones are marked by the GFP expression (arrowheads). (B–F′) Wing discs containing smo³ clones that express Myc-Smo (B-B′), Myc-Smo^K851R (C-C′), Myc-Smo^K851R-SUMO (D-D′), Myc-Smo^SD123 (E-E′), or Myc-Smo^{SD123 K851R} (F-F′) driven by the C765 Gal4 driver were immunostained with Myc, Ci, En or Ptc antibodies. smo³ clones expressing Smo transgenes are marked by the Myc expression. Arrows and arrowheads indicate clones distant from or close to the A/P compartment boundary, respectively. See also Figure S4.