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. Author manuscript; available in PMC: 2017 Nov 21.
Published in final edited form as: Dev Cell. 2016 Oct 13;39(4):438–451. doi: 10.1016/j.devcel.2016.09.014

Figure 6. Sumoylation of Smo recruits UBPY to antagonize its ubiquitination.

Figure 6

(A–B) Cell based ubiquitination assays to determine the effect of blocking sumoylation on Smo ubiquitination. (C) Schematic drawing of UBPY with the regulatory domain (RD) and catalytic domain (CD) represented by filled boxes. The arrows indicate the position of the putative SUMO-interacting motifs (SIM). (D) Western blot analysis to show that Hh stimulates UBPY/Smo association through Smo sumoylation. (E) Ubiquitination assay of extracts from S2 cells transfected with the indicated constructs. (F–G′) Wing discs expressing Fg-UBPY (F-F′) or Fg-UBPY-SIM (G-G′) were immunostained with Flag (Fg) and Smo antibodies. (H) Hh inhibits Smo ubiquitination and degradation through stimulating its sumoylation. See also Figure S7.