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. Author manuscript; available in PMC: 2017 Nov 21.
Published in final edited form as: Curr Biol. 2016 Nov 10;26(22):2981–2991. doi: 10.1016/j.cub.2016.08.072

Figure 1. tph1b Regulatory Sequences Permit Clonal Analysis of Blastemal Cells.

Figure 1

(A and B) tph1b:mCherry reporter expression in regenerating fins at 2 (A) and 5 (B) days post amputation (dpa).

(C) Section of tph1b:mCherry fin at 2 dpa.

(D) Design of lineage tracing experiments in (E and F).

(E and F) tph1b:CreER; ubi:switch fins were treated with 4 μM tamoxifen from 24-36 hours post amputation (hpa) and imaged at 5 (E) and 20 (F) dpa.

(G) Section through 3 dpa lineage-labeled tph1b:CreER; ubi:switch fin, stained for osteoblasts (green) using the Zns5 antibody as well as nuclei (DAPI, blue). e, epidermis; o, osteoblasts, if, intraray fibroblasts.

(H) Design of clonal analysis experiments in (I to K).

(I) Representative image of a single labeled blastemal cell (arrow) in a 2 dpa blastema.

(J) Two-D projection of all initial labeling events onto a rescaled virtual blastema, with rescaled lateral coordinates (D, dorsal; V, ventral) and initial PD coordinates (amputation plane, P, proximal; distal tip, D, distal), color-coded by time of detection of the initial labeling event.

(K) Two-D projection of all initial labeling events onto a rescaled virtual blastema, color-coded by ray of origin (1 indicates lateral-most ray, 8 indicates the medial ray). Dashed lines, amputation planes.

See also Figure S1.