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Western blotting was used to determine the expression of Smad2/3 by rhesus and human PBMC treated with rhTGFβ1 (10 ng/mL) or TGFβ1/Fc (5 μg/mL) for 30 min. The same cell lysates were used to evaluate the expression of GAPDH as a loading control. One representative experiment of two performed is shown. PBMC, peripheral blood mononuclear cell.
