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. Author manuscript; available in PMC: 2017 Oct 1.

Published in final edited form as: Am J Transplant. 2016 Jul 11;16(10):2994–3006. doi: 10.1111/ajt.13883

Rhesus monkey PBMC were stimulated with anti-CD3 and anti-CD28 Abs and cultured without or with rapamycin (1 ng/mL) ± the indicated concentrations of TGF-β1/Fc (0, 1, 10 μg/mL) and with or without exogenous IL-2. On day 4, the cells were harvested and further activated with PMA, ionomycin and GolgiStop for 4 h, then stained for intracellular IL-17 and Foxp3. (A) Representative percentages of CD4⁺CD25⁺Foxp3⁺ Treg. (B) Overall Treg percentages and (C) Treg/Th17 ratios plotted as means ± SD. n=5; **, p<0.001; *, P<0.01.

Rhesus monkey PBMC were stimulated with anti-CD3 and anti-CD28 Abs and cultured without or with rapamycin (1 ng/mL) ± the indicated concentrations of TGF-β1/Fc (0, 1, 10 μg/mL) and with or without exogenous IL-2. On day 4, the cells were harvested and further activated with PMA, ionomycin and GolgiStop for 4 h, then stained for intracellular IL-17 and Foxp3. (A) Representative percentages of CD4⁺CD25⁺Foxp3⁺ Treg. (B) Overall Treg percentages and (C) Treg/Th17 ratios plotted as means ± SD. n=5; **, p<0.001; *, P<0.01.