Figure 1.

Mast cell deficiency in the Kit-independent Mcpt5-Cre R-DTA model does not affect body weight, glucose tolerance, and insulin resistance in diet-induced obesity. (A,B) Mast cell-deficient Mcpt5-Cre⁺R-DTA⁺ and mast cell-proficient Cre-negative R-DTA⁺ littermate control male mice were fed a HFD and weight (A) and weight gain (B) were recorded. (C,D) Glucose tolerance test (C) and insulin tolerance test (D) were performed in mast cell-deficient Mcpt5-Cre⁺R-DTA⁺ and mast cell-proficient Cre-negative R-DTA⁺ littermate mice subjected to diet-induced obesity. In (D), data are presented as percentage of initial glucose level. Data and corresponding area under curve (AUC) analysis are shown. AUC data are presented as % of control; the Cre-negative group was set as the 100% control. AUC data were tested for statistical significance with Student’s t-test. Data are expressed as means ± SEM (n = 8–10 mice).