Figure 3.

No difference in AT inflammation between obese mast cell-deficient and -proficient mice. (A–H) The stromal vascular fraction (SVF) of subcutaneous (sAT) and gonadal (gAT) adipose tissue from obese mast cell-deficient and -proficient mice was analyzed by flow cytometry for the accumulation of total macrophages (Mφs) and pro-inflammatory M1-polarized macrophages. The presence of CD3⁺ T lymphocytes and of T helper CD4⁺ and cytotoxic CD8⁺ T cells was analyzed. Student’s t-test was used for statistical analysis; data are expressed as means ± SEM (n = at least 8 mice/group). (A,B) Percentual analysis of macrophage accumulation in the sAT (A) and gAT (B). (C,D) Number of macrophages per gram of sAT (C) and gAT (D). (E,F) Percentual evaluation of T cell accumulation in the sAT (E) and gAT (F). (G,H) Number of T cells per gram of sAT (G) and gAT (H). (I,J) Representative images (Giemsa staining) for the presence of mast cells in the gAT of obese mast cell-deficient and -proficient mice (I). Mast cell deletion was evaluated (J); n = at least 8 mice/group. Mast cells were not present in the AT of Mcpt5-Cre⁺R-DTA⁺ mice. *p < 0.05. (K) Quantitative PCR analysis of inflammatory gene expression in the gAT of obese mast cell-deficient and -proficient mice. Data are shown relative to the mast cell-proficient mouse group; 18S RNA was used for normalization. Mann–Whitney U test was used for statistical analysis (n = 8 mice/group).