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. 2016 Oct 18;6:153–158. doi: 10.1016/j.bbacli.2016.10.003

Fig. 3.

Fig. 3.

Whole cell and mitochondrial state3 respiration, APTase6/8 subunits expression, complex V activity and serotonin levels in control and ADHD cybrids: Whole cell respiration (A), cellular rate of oxygen consumption per 3 × 106 cells (B), mitochondrial State3 respiration (C), and mitochondrial oxygen consumption rate per 3 × 106 cells in ADHD and control cybrids (D). The cDNA prepared from total RNA of ADHD and control cybrids were subjected to qRT-PCR analysis using specific primers to accurately measure the transcript level of ATPase6/8 (E, F). Levels of serotonin in differentiated control and ADHD cybrid-neurons (G). Complex V activity using BN-PAGE, Activity gel (H), Coomassie staining gel (I). Bar diagram shows the difference in complex V activity analysed by Image software (J). (Control samples- 1 to 4 lanes and ADHD samples- 5 to 7 lanes in both the gels, M lane is marker). Data are presented as Mean ± SEM. Values of p* ≤ 0.05 is considered significant.