Fig. 2.

Lyve-1 expression in dendritic cells, high endothelial venules (HEVs) and LECs. a Lymph node (ln) located between the parotis (pa) and the submandibular gland (smg). Magnification ×40. Bar 200 μm. b Lymph node showing Lyve-1 expression in HEVs (hev). Magnification ×200. Bar 50 μm. c Anti-podoplanin staining of the same lymph node as that in b. Note the podopanin⁺ reticular cells and podoplanin⁺ sinuses. Magnification ×100. Bar 100 μm. d Immunofluorescence staining of a lymph node with the antibodies anti-Lyve-1 (green) and anti-podoplanin (red). Note the Lyve-1⁺/Podoplanin⁺ visceral layer (vl) and the Lyve-1⁻/Podoplanin⁺ parietal layer (pl). Magnification ×400. Bar 20 μm