Fig. 3.

Lyve-1-positive lymphatics in the basal parts of the nasal mucous membrane and along the nasolacrimal duct. a Overview of the nasal mucous membrane showing the position of the vomeronasal organ (arrowheads) and the nasolacrimal duct (arrows). Magnification ×20. Bar 400 μm. b Lymphatics associated with the vomeronasal organ. Magnification ×100. Bar 100 μm. c Lymphatic plexus around the nasolacrimal duct. Magnification ×100. Bar 100 μm. d Lymphatics in the basal part of the nasal mucous membrane (ns nasal septum, pa palatine, to tongue). Magnification ×40. Bar 200 μm. e, f Immunofluorescence double-staining of lymphendothelial markers Lyve-1 (e, green) and Podoplanin (f, red) of regions near the nasolacrimal duct. Note the Lyve-1⁺/Podoplanin⁺ lymph vessels (lv) and Lyve-1⁻/Podoplanin⁻ blood vessels (bv). Magnification ×400. Bar 20 μm