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. 2016 Sep 6;366(3):667–677. doi: 10.1007/s00441-016-2493-8

Fig. 4.

Fig. 4

Lymphatics are absent in the upper parts of the nasal mucous membrane. a–c Immunofluorescence double-staining (green anti-Lyve-1, red anti-Podoplanin, blue 4,6-diamidino-2-phenylindole [Dapi]) of the lower part of the nasal mucous membrane. Note the epithelial layer (e in a, d) on top and Lyve-1+/Podoplanin+ lymph vessels (lv). Magnification ×400. Bar 20 μm. d–f Immunofluorescence double-staining (green anti-Lyve-1, red anti-Podoplanin, blue Dapi) of the upper part of the nasal mucous membrane. No double-positive lymph vessels can be detected. Note the Lyve-1/Podoplanin+ basal cell layer (bc in c, f). Magnification ×400. Bar 20 μm