Fig. 7.

Lyve-1-positive lymphatics of the dura mater. a Telencephalic hemispheres and superior sagittal sinus (sss). Lyve-1-positive structures rarely show a distinct lumen. b Lyve-1-positive structures in deeper aspects of the telencephalic hemispheres. a, b Magnification ×200. Bar 50 μm. c–e, f–h Immunofluorescence double-staining (green anti-CD31, red anti-Prox-1, blue Dapi) of cryo-preserved mouse brains (day 14). c–e Example of Prox-1⁺ endothelial cell nucleus embedded in a CD31⁺ vessel located in the dura mater. f–h Example of Prox-1⁺ endothelial cell nucleus embedded in a CD31⁺ vessel located in deeper aspects of the telencephalic hemispheres. c–h Magnification ×400. Bar 20 μm