FIGURE 4.

Effects of NaCl and mannitol stresses on SiLTP transgenic tobacco seed germination. Expression levels of SiLTP in transgenic tobacco and WT plants (A). NaCl or mannitol sensitivity of WT and transgenic plants (B). Seeds of WT and transgenic lines were germinated and then grown for 8 days on medium independently supplemented with 0 (control), 100 mM NaCl, 250 mM mannitol, and 300 mM mannitol. The corresponding seed germination rates (C), root lengths (D), and shoot lengths (E) are compared. ^∗, ^∗∗, and ^∗∗∗ indicate statistically significant differences at P < 0.05, P < 0.01, and P < 0.001 (Student’s t-test), respectively.