FIGURE 6.

Salt resistance analysis of T1 transgenic and WT tobacco seedlings. Four-week-old seedlings of the transgenic lines and WT were irrigated for 0, 10, and 28 days with 250 mM NaCl (A); the survival rates of transgenic and WT seedlings after watering for 28 days with NaCl (B); the proline content (C); and soluble sugar content (D) were analyzed in transgenic and WT plants after watering for 21 and 28 days with NaCl, respectively. Six seedlings of each transgenic line and WT were planted in each small basin for the NaCl treatment and corresponding analysis; 18 plants were used in each experiment. ^∗∗ and ^∗∗∗ indicate statistically significant differences at P < 0.01 and P < 0.001 (Student’s t-test), respectively.