Figure 6.

qRT-PCR analysis of tissue-specific relative expression levels of insulin-like peptide receptor 1 (InR-1) across castes of B. terrestris. InR-1 is a receptor involved in insulin signaling. It is upregulated in virgin and diapausing queens, especially in crop and hypopharyngeal glands (see main text). Data represent mean ± SEM (n = 3–5) of normalized and rescaled expression levels. (A) Heatmap: Log-scale. Bar graphs: Relative transcript levels in linear scale (B) and log-transformed scale (C). Significantly different expression levels are indicated by different letters (p < 0.05).