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. 2016 Nov 24;7:574. doi: 10.3389/fphys.2016.00574

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Copyright © 2016 Jedlička, Ernst, Votavová, Hanus and Valterová.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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qRT-PCR analysis of tissue-specific relative expression levels of forkhead box protein O (FOXO) across castes of B. terrestris. FOXO is a transcription factor involved in stress tolerance, diapause, longevity, and growth. FOXO transcription is lower in workers and reproducing queens than in other castes (see main text). Data represent mean ± SEM (n = 3–5) of normalized and rescaled expression levels. (A) Heatmap: Log-scale. Bar graphs: Relative transcript levels in linear scale (B) and log-transformed scale (C). Significantly different expression levels are indicated by different letters (p < 0.05).