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. 2016 Nov 24;10:254. doi: 10.3389/fncel.2016.00254

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Copyright © 2016 Zhang, Gao, Sun, Xu, Chen, Sun and Wu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution and reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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RGC apoptosis increased after transfection of lenti-shPOLG. (A) Terminal deoxynucleotidyl transferase dUTP nick end-labeling (TUNEL) assay for detecting apoptotic cell death. Representative microscopic images showing TUNEL-positive cells in the RGCs treated with lenti-shPOLG or with lenti-scrambled shRNA. Red, TUNEL; blue, DAPI. Scale bars, 50 μm. (B) Quantitative analysis of RGC apoptosis determined by TUNEL assay. The data are expressed as survival cell counts. (C) The active form of caspase-3 increased as determined by western blot using an antibody to cleaved caspase-3. Values are presented as the means ± SEMs. **P < 0.01, ***P < 0.001, compared with control RGCs transfected with lenti-scrambled shRNA. Sh, lenti-shPOLG-transfected RGCs; SC, lenti-scrambled shRNA-transfected RGCs.