Table 2.
PCR conditions used in this study.
| Temp (°C) |
Rate (Δ°/cycle) |
Time |
Cycles |
|---|---|---|---|
| 95 | 5 m | ||
| 94 | 30 s | ||
| 62 | −0.5 | 45 s | 10 |
| 72 | 1 m | ||
| 94 | 30 s | ||
| 57 | 45 s | 25 | |
| 72 | 5 m | ||
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