Table 2. Number of surviving colonies on selective 3-AT plates using B1H assay.
| Efficiency |
3-AT [mM] |
||||
|---|---|---|---|---|---|
| 2 | 3 | 4 | |||
| a | pB1H1+pH3U3 lib | 108 | 0 | 0 | 0 |
| b | pB1H1-FGF14+pH3U3 lib | 108 | 1,200 | 750 | 550 |
| c | pB1H1-FGF14(186–191)+pH3U3 lib | 108 | 8 | 3 | 0 |
Survival represents DNA binding. (a) A plasmid without FGF14 (pB1H1) does not bind DNA. (b) A plasmid with FGF14 shows DNA binding activity. (c) FGF14 in which four residues that were predicted by Dr PIP to be in the binding site were mutated, yielded a negligible number of colonies suggesting virtual abrogation of binding.