Fig. 1.

ELISA for anti-hGAA IgG antibody in GAA-KO mice under weekly ERT with 20 mg/kg of rhGAA. The antibody levels were presented by the absorbance for 1:200 dilution of plasma from the indicated groups of mice. Mean plus standard deviation  is shown, n = 10 for each group. (A) Both the 3-dose anti-CD4 mAb (anti-CD4x3 group) and methotrexate treatments significantly (p < 0.05) reduced rhGAA antibody levels in the GAA-KO mice after 4 weeks of rhGAA treatment in comparison with the control mice (ERT only). (B) A 2-dose treatment (anti-CD4x2 group) was similarly effective in inhibiting anti-rhGAA antibody formation as the 3-dose regimen (anti-CD4x3 group), but a single-dose treatment (anti-CD4x1 group) failed to significantly (p > 0.05) reduce the anti-rhGAA antibody levels, in comparison to the control group.