TABLE 2.
Details of C. coli isolates with atypical mapA/ceuE primer and probe sequences
| Atypical target and isolate | ST (CC)a | Gene allele/primer and probe combination (RT-PCR result)b |
mapA/ceuE RT-PCR result | |
|---|---|---|---|---|
| mapA | ceuE | |||
| mapA | ||||
| OXC7352 | 6973 (ST1150) | 20/2 (+) | 136/22 (+) | Mixed |
| OXC6987 | 825 (ST828) | 88/1 (+) | 3/22 (+) | Mixed |
| OXC6395 | 1487 (ST1150) | 19/22d (−) | 17/22 (+) | C. coli |
| OXC7615 | 6760 (UAc) | 111/23e (late +) | 139/22 (+) | Inconclusive |
| ceuE | ||||
| OXC7241 | 6698 (UA) | 96/19 (−) | 153/25 (late +) | Inconclusive |
| OXC7243 | 6698 (UA) | 96/19 (−) | 153/25 (late +) | Inconclusive |
| OXC7653 | 6975 (UA) | 114/21 (−) | 183/26 (late +) | Inconclusive |
a
CC, clonal complex.
b
+, target detected; −, target not detected; late +, target detected after cycle 30.
c
UA, ST not assigned to a clonal complex.
d
Forward primer C. jejuni specific, probe and reverse primer C. coli specific.
e
Forward primer and probe C. jejuni specific, reverse primer C. coli specific.