TABLE 3.

Overview of standardization results of the 12 clinical strains belonging to 11 fungal species included in the new panfungal qPCR assay^a

Species	FAM (panfungal channel)				HEX (Mucorales channel)				ROX (Aspergillus sp. channel)				Cy5 (endemic mycosis fungus channel)
	S	R2	CV (%)	DL (fg/μl)	S	R2	CV (%)	DL (fg/μl)	S	R2	CV (%)	DL (fg/μl)	S	R2	CV (%)	DL (fg/μl)
Rhizopus oryzae	−3.6	0.99	2.45	0.1	−3.6	0.99	2.05	0.1
Rhizopus microsporus	−3.6	0.98	1.54	0.1	−3.8	0.98	3.05	0.1
Mucor circinelloides	−3.4	0.98	3.76	0.1	−3.7	0.98	4.67	1
Aspergillus fumigatus	−3.5	0.97	2.72	1					−4.1	0.95	3.73	1
Aspergillus flavus	−3.8	0.98	3.69	1					−4.1	0.98	3.05	1
Aspergillus terreus	−3.5	0.98	1.52	1					−4.1	0.98	3.6	1
Histoplasma capsulatum var. capsulatum	−3.5	0.98	2.23	1									−3.5	0.98	1.98	1
Histoplasma capsulatum var. duboisii	−3.4	0.98	2.21	10									−3.3	0.98	2.16	10
Coccidioides immitis	−3.5	0.96	2.9	10									−4.6	0.96	4.4	10
Coccidioides posadasii	−5.1	0.95	3.98	10									−6.6	0.97	5.69	100
Blastomyces dermatitidis	−3.4	0.98	1.93	1									−3.2	0.98	1.67	1
Paracoccidioides brasiliensis	−2.8	0.98	0.73	1									−2.8	0.98	0.47	1

^aS, slope of standard curve; R², coefficient of determination; CV, coefficient of variation; DL, detection limit.