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. 2016 Nov 22;7:13473. doi: 10.1038/ncomms13473

Figure 1. Introduction of glycan probes to MERS-CoV RBD vaccine.

Figure 1

(a) Crystal structure of MERS-CoV RBD (PDB access code: 4L3N). The core structure is coloured in cyan, and the receptor-binding motif (RBM) in red. Four residues are shown where an N-linked glycan probe was introduced. (b) Structure of MERS-CoV RBD complexed with human DPP4 (PDB access code: 4KR0), showing the role of the four epitopes in the binding of the RBD to DPP4. (c) AlphaScreen assay was performed to detect the binding between recombinant MERS-CoV RBDs and recombinant human DPP4. PBS buffer was used as a negative control. Binding affinity was characterized as AlphaScreen counts. (d) Fluorescence-activated cell sorting (FACS) was carried out to detect the binding between recombinant MERS-CoV RBDs and cell-surface-expressed human DPP4. Human IgG protein was used as a negative control. Binding affinity was characterized as median fluorescence intensity. Error bars indicate s.e.m. ***: P<0.001.