Table 1.

Crystallization conditions for different models of AlDHPyr1147.

Crystals	Composition of protein solution	Composition of reservoir solution
Apo form (Apo)	17.7 mg/mL apo form (0.32 mM of monomer) in 50 mM HEPES, pH 7.5, containing 50 mM NaCl and 5 mM β-ME	0.1 М imidazole, pH 6.5, and 1.0 M sodium acetate trihydrate
AlDHPyr1147 as isolated (Holo-1)	17 mg/mL AlDHPyr1147 as isolated in 50 mM HEPES, pH 7.5, containing 50 mM NaCl and 5 mM β-ME	0.01 M nickel(II) chloride, 0.1 M Tris, pH 8.5, 1.4 M lithium sulfate, and 50 mM NaCl
Complex with NADP+ (Holo-2)	Cocrystallization of 17.7 mg/mL apo form with 27 mM NADP+ in 50 mM HEPES, pH 7.5, containing 50 mM NaCl and 5 mM β-ME	0.1 М imidazole, pH 6.5, and 1.0 M sodium acetate trihydrate
Complex with NADP+ (Holo-3)	Soaking crystals of the apo form in excess of NADP+ and isobutyraldehyde for 40 min at 25°C
Ternary complex with NADP+ and substrate (ternary complex)	Cocrystallization of 17.7 mg/mL apo form with 27 mM NADP+ and 400 mM isobutyraldehyde in 50 mM HEPES, pH 7.5, containing 50 mM NaCl and 5 mM β-ME	0.1 М imidazole, pH 6.5, and 1.0 M sodium acetate trihydrate