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(a) PCR-RFLP products with SspI restriction enzyme. Three cuttings in locations 108, 258, and 421 bp are visible on gel electrophoresis. (b) PCR-RFLP products with VspI restriction enzyme. Three cuttings in locations 104, 106, and 600 bp are visible on agarose gel. Lane M, DNA size marker. Lane 1, positive control for Cryptosporidium. Lanes 2–4, positive Cryptosporidium samples.
