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. 2016 Oct 6;5(10):e004028. doi: 10.1161/JAHA.116.004028

Figure 3.

Figure 3

Inhibitory effects of ultrasound‐targeted microbubble destruction (UTMD)–mediated G protein–coupled receptor kinase type 4 (GRK4) small interfering RNA (siRNA) delivery in renal GRK4 expression. A, Ultrasound image of the kidney of a spontaneously hypertensive rat (SHR) (42 frames per s, mechanical index=0.9, frequency=7.00 MHz). B‐mode scans, gray‐scale mapping. The images were obtained at 0 seconds (a), 15 seconds (b), and 5 minutes (c) after the injection of the microbubbles. B and C, Renal GRK4 mRNA and protein expression levels after treatment with UTMD‐mediated GRK4 siRNA delivery for 20 days in SHRs (n=5, *P<0.05 vs control). D and E, Effects of UTMD‐mediated GRK4 siRNA delivery on GRK4 expression in the heart and mesenteric arteries of SHRs after treatment with UTMD‐mediated GRK4 siRNA delivery for 20 days (n=5).