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. 2016 Oct 6;5(10):e004117. doi: 10.1161/JAHA.116.004117

Figure 4.

Figure 4

LMO2 knockdown (KD) impaired endothelial network formation, which can be rescued by exogenous transforming growth factor‐β1 (TGF‐β1). A, Network formation of control (CT) and LMO2 KD human umbilical vein endothelial cells (HUVECs). B, List of downregulated genes identified in LMO2 KD in comparison to CT HUVECs using angiogenesis polymerase chain reaction (PCR) array. C, Reverse transcription PCR (RT‐PCR) of TGF‐β1. D, ELISA for TGF‐β levels in culture medium of CT and LMO2 KD cells. E, RT‐PCR of TGF‐β1 in CT and LMO2 overexpression HUVECs. F, Network formation of CT and LMO2 KD HUVECs with or without supplementation of TGF‐β 10 ng/mL. G, Chromatin immunoprecipitation PCR analysis of LMO2 complex binding to TGF‐β1 promoter (–901 and –4030 region) in both CT and LMO2 KD cells. Data are presented as mean±SEM (n=3). * P<0.05 vs CT.