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. 2016 Oct 11;5(10):e004340. doi: 10.1161/JAHA.116.004340

Figure 2.

Figure 2

Roles of macrophages and T lymphocytes in intracerebral hemorrhage (ICH)–induced inflammation. A, Absolute numbers of infiltrating macrophages on day 1 and day 4 after ICH. B, Absolute numbers of infiltrating T lymphocytes on day 4 after ICH in clodronate liposomes (CLP)–treated or liposome (vehicle)‐treated mice. Data were obtained for samples pooled from 5 mice, and the experiments were repeated 3 times. **P<0.01 vs vehicle. C, Neurologic deficit score (NDS) at 1, 4, and 7 days after ICH in the CLP‐ and vehicle‐treated mice. *P<0.05 vs vehicle, n=6 per group. D, Brain water content (BWC) at 1, 4, and 7 days after ICH in the CLP‐ and vehicle‐treated mice. *P<0.05 vs vehicle, n=4 per group. Two‐way ANOVA reported a significant difference in main effects of all treatment groups (P<0.05) but not of time points (P>0.05), there was no interaction between treatments and time points (P>0.05). E and F, Absolute numbers of infiltrating macrophages and T lymphocytes on day 4 after ICH in fingolimod‐treated or untreated mice. Data were obtained for samples pooled from 5 mice, and the experiments were repeated 3 times. *P<0.05 vs vehicle. G, NDS for vehicle (dimethyl sulfoxide [DMSO])‐, fingolimod‐, and fingolimod+R‐treated mice at 1, 4, and 7 days after ICH. *P<0.05 vs vehicle, n=6 per group. Two‐ANOVA reported a significant difference in main effects of all treatment groups (P<0.05) but not of time points (P>0.05), there was no interaction between treatments and time points (P>0.05). H, BWC for vehicle (DMSO)‐, fingolimod‐, and fingolimod+R‐treated mice at 1, 4, and 7 days after ICH. *P<0.05 vs vehicle, n=4 per group. I, NDS for wild‐type (WT), Rag1−/−, and Rag1−/− mice that received CD3+ T lymphocytes from WT mice (WT CD3→Rag1−/−) at 1, 4, and 7 days after ICH. # P<0.05 vs WT mice, *P<0.05 vs Rag1−/− mice, n=5. Two‐way ANOVA reported significant difference in main effects of genotype (P<0.05) but not of time points (P>0.05), there was no interaction between genotypes and time points (P>0.05). J, BWC for WT, Rag1−/−, and WT CD3→Rag1−/− mice at 1, 4, and 7 days after ICH. # P<0.05 vs WT mice, *P<0.05 vs Rag1−/− mice, n=5.