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. 2016 Nov 24;6:37450. doi: 10.1038/srep37450

Figure 5. Endothelial cell colony forming units (EC-CFUs) from malignant breast disease patients were resistant to TNF-α-induced apoptosis.

Figure 5

(A) The EC-CFUs were treated with a vehicle or TNF-α at 1 and 10 pg/mL for three hours. Caspase 3 activity was measured using a caspase-3 fluorogenic substrate (Ac-DEVD-AFC) by enzyme-linked immunosorbent assay. The relative activity of caspase 3 was normalised to that which received the vehicle treatment. (B) The active caspase 3-posive cells from 6 healthy controls and 8 malignant breast disease patients were detected using fluorescence microscopy and quantified 24 hours after the administration of TNF-α. (C) DiI-Ac-LDL-positive cells from 3 healthy controls and 3 malignant breast disease patients were detected using fluorescence microscopy and quantified 24 hours after the administration of TNF-α. The relative activity of caspase 3/DiI-Ac-LDL uptake in cells with TNF-α treatment was normalised to that which received the vehicle treatment. The mean of relative activity was obtained by averaging independent participants. The representative pictures of caspase 3- or DiI-Ac-LDL-positive cells were taken at 40X magnification (lower panel). Bar, SE; *p < 0.05; **p < 0.01.