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. 2016 Nov 24;6:37467. doi: 10.1038/srep37467

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Copyright © 2016, The Author(s)

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ROS activity was measured by fluorescent-labeled DHE staining. 2K1C rats had stronger fluorescence intensity labeled with DHE compared with SHAM rats. ExT significantly decreased DHE staining in the PVN of 2K1C rats. (a) A representative immunofluorescence image of DHE. (b) Densitometric analysis of immunofluorescent intensity of DHE in the PVN in different groups. DHE: dihydroethidium; PVN: hypothalamic paraventricular nucleus; 2K1C: two-kidney, one-clip; ExT: exercise training; Sed: sedentary. Values are expressed as means ± SE. *P < 0.05 vs SHAM groups (SHAM + Sed or SHAM + ExT); ^†P < 0.05 2K1C + ExT vs 2K1C + Sed.