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. 2016 Nov 24;6:37695. doi: 10.1038/srep37695

Figure 9. CXCL1 and CXCL2 are positive modulators of mycobacteria induced production of inflammatory molecules.

Figure 9

(a–c) CXCL1 and CXCL2 were neutralized by using 20 μg of CXCL1 and CXCL2 neutralizing antibody, respectively in vivo to assess the status of Mtb H37Ra induced production of pro-inflammatory cytokines TNF-α, IL-12p40 and IL-1β in lymph node (a), lung (b) and spleen (c) of mice by ELISA. (d–i) The role of CXCL1 and CXCL2 in regulating the Mtb H37Ra induced production of inflammatory mediators such as defensins in lymph node (d), lung (e), spleen (f) and Nox2, Nos2, Lyz in lymph node (g), lung (h) and spleen (i) were also assessed by qRT-PCR. All the data represent means ± SE (N = 3), *p < 0.05, **p < 0.005, ***p < 0.0001 (One way Anova or t test). Neu, neutralizing; Ab, antibody; pg, picogram; PBS, phosphate buffered saline.