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. 2016 Nov 24;6:37697. doi: 10.1038/srep37697

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) Microarray results for the top 35 downregulated genes in EBs treated with ATV during days 1–4. Data were compared with control EBs and analysed on days 3 and 4. Gene expression levels in ATV-treated EBs are expressed as Log2 (fold change) values relative to control EBs. (b) In situ hybridisation to detect T and Sox2 in EBs treated with or without ATV on days 1–6 and collected at the indicated times. Results are representative of >100 EBs/group. (c) Real-time PCR of T and Sox2 in EBs treated with or without ATV and/or MVA during days 1–4 and collected on day 4. Results were analysed as in Fig. 1b. *P < 0.05, **P < 0.0001.