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. 2016 Nov 24;6:37697. doi: 10.1038/srep37697

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) Heat map showing differences in the profiles of 147 metabolites in EBs that were treated with or without ATV during days 3–5 and subjected to metabolomic analysis on days 4 and 5. (b) Principal component analysis of the EBs in (a). Principal components 1 and 2 account for 36.9% and 29.5% of total variance, respectively. (c) Intracellular levels of the indicated metabolites in the EBs in (a). Data are expressed as the relative area of signal peaks corresponding to each metabolite and represent the mean ± SD (n = 3). (d) Intracellular levels of the indicated free FAs and acylcarnitines in the EBs in (a) analysed as in (c). (e) Intracellular cholesterol levels in the EBs in (a) analysed as in (c). *P < 0.005.