Table 3. Percentage taxonomic assignments of reads averaged across each species and the series of blanks determined using MEGAN.

	Barley	Maize	Grape	Rice	Blanks
Bacteria	55.66	67.25	72.26	42.92	9.36
Eukaryotes	4.06	8.06	4.59	1.82	5.74
Plants	0.65	1.62	2.04	0.36	0.19
Target	0.01	0.14	0.16	0.03	0.00
Not assigned	39.61	22.94	20.95	54.86	84.70

Results indicate assignment prior to PIA filtering.