| TOP10 |
F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 Δ lacX74 recA1 araD139 Δ(araleu)7697 galU galK rpsL (StrR) endA1 nupG |
Invitrogen |
| NEB® 5-alpha |
fhuA2 Δ(argF-lacZ)U169 phoA glnV44 Φ80 Δ(lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17 |
New England Biolabs |
| BL21 (DE3) |
fhuA2 [lon] ompT gal (λ DE3) [dcm] ∆hsdS λ DE3 = λ sBamHIo ∆EcoRI-B int::(lacI::PlacUV5::T7 gene1) i21 ∆nin5
|
Invitrogen |
| Plasmid |
Relevant features |
Source |
| pUC19 |
E. coli cloning vector; Apr
|
Thermo Scientific |
| pCR-BluntII-TOPO |
E. coli cloning vector; Kmr, Zeor
|
Invitrogen |
| pGEX-6P-1 |
E. coli expression vector, PreScission Protease cleavage site between N-terminal GST tag and target protein; Apr
|
GE Healthcare |
| pVA2198 |
E. coli-Bacteroides shuttle vector, source of ermF-ermAM cassette; Spr
|
55 |
| pT-COW |
E. coli-Bacteroides shuttle vector, Tcr
|
58 |
| p1604AeB-D |
Plasmid for porZ deletion mutagenesis, derivative of pUC19 |
This study |
| p291AeB-C |
Plasmid for porN deletion mutagenesis, derivative of pUC19 |
This study |
| pPorU/pUC19/Erm |
Plasmid for porU deletion mutagenesis, derivative of pUC19 |
This study |
| pT-COW-porZ |
Plasmid for porZ complementation, derivative of pT-COW |
This study |
| p1604CeB-H |
Master plasmid for PorZ modifications; derivative of pUC19 |
This study |
| pAT1 |
Plasmid for Q678i6H mutagenesis in PorZ, derivative of p1604CeB-H |
This study |
| pAG1 |
Plasmid for S683 > 6 H mutagenesis in PorZ, derivative of p1604CeB-H |
This study |
| pAL5 |
Plasmid for I770i6H mutagenesis in PorZ, derivative of p1604CeB-H |
This study |
| pAL11 |
Plasmid for A686 > 6 H mutagenesis in PorZ, derivative of p1604CeB-H |
This study |
| pAL12 |
Plasmid for L689 > 6 H mutagenesis in PorZ, derivative of p1604CeB-H |
This study |
| pAL13 |
Plasmid for D690i6H mutagenesis in PorZ, derivative of p1604CeB-H |
This study |
| p1604M1 |
Plasmid for I770 > 6 H mutagenesis in PorZ, derivative of p1604CeB-H |
This study |
| p1604M2 |
Plasmid for F677i8H mutagenesis in PorZ, derivative of p1604CeB-H |
This study |
| p1604M3 |
Plasmid for R776i8H mutagenesis in PorZ, derivative of p1604CeB-H |
This study |
| pPorU-E |
Master plasmid for PorU modification, derivative of pUC19 |
This study |
| pPorU/C690A |
Plasmid for C690A mutagenesis in PorU, derivative of pPorU-E |
This study |
| pGEX-6P-1/PorZ |
Plasmid for PorZ purification from E. coli, derivative of pGEX-6P-1 |
This study |
