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. 2016 Nov 24;6:37717. doi: 10.1038/srep37717

Figure 2. Knockdown of SATB1 ameliorates CCl4-induced fibrosis in HBV-Tg mouse model.

Figure 2

(a) Adenovirus carrying shRNA against SATB1 (AdshSATB1) or negative control (AdshNC) was injected into HBV-Tg mouse model. Inhibition efficiency of SATB1 expression was examined by real-time PCR in the fibrotic livers from AdshSATB1 or AdshNC-treated mice (n = 6 in each group). **P < 0.01 vs. AdshNC group. (b) Western blot was used to detect the protein levels of SATB1 and α-SMA in liver tissues (n = 3 in each group). Full-length blots are included in the Supplementary Fig. S6. (c) Representative IHC staining was used to determine the expression of α-SMA in the fibrotic liver. H&E and Masson’s trichrome staining were used to show pathological conditions and collagen deposition (100×). (d) Plasmid levels of ALT and AST were detected by elisa Kit (n = 6). *P < 0.05 vs. AdshNC group. (e) Semi-quantitative analysis of Masson’s trichrome staining in the fibrotic livers from AdshSATB1 or AdshNC-treated mice (n = 6). *P < 0.05 vs. AdshNC group. (f) Real-time PCR was used to detect the expression of SATB1, α-SMA, COL1A1, IL-6, CTGF in liver tissues from HBV-Tg mice. (n = 6). *P < 0.05, **P < 0.01 vs. AdshNC group.