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. 2016 Nov 24;6:37717. doi: 10.1038/srep37717

Figure 3. HBx induces SATB1 expression in hepatic cell line L02.

Figure 3

(a) Real-time PCR and western blot were used to examine SATB1 expression from L02 cells respectively transfected with synthetic DNA plasmids containing seven viral genes of HBV. *P < 0.05, **P < 0.01 vs. Vector group. Full-length blots are included in the Supplementary Fig. S7. (b) Identification of HBx from stable HBx-transfected L02 (L02-HBx) and Chang liver cells (CHL-HBx) and corresponding vector control (L02-Vector, CHL-Vector), widetype control (WT) by agarose gelelectrophoresis. Full-length gels are included in the Supplementary Fig. S7. (c) The mRNA levels of SATB1 and TGFB1 were analyzed by real-time PCR. (n = 3). *P < 0.05 vs. Vector. (d) The expression of SATB1 was analyzed by western blot. Full-length blots are included in the Supplementary Fig. S7. *P < 0.05, **P < 0.01 vs. Vector. (e) HBx trans-activated SATB1 promoter activity. SATB1 promoter plasmids were transfected into L02-HBx, CHL-HBx and corresponding control groups. (n = 3). **P < 0.01 vs. Vector.