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. 2016 Nov 24;6:37801. doi: 10.1038/srep37801

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Upper panel is the phase contrast image of the field shown on the below panel (A). Tubular structures were photographed (A and B) and then quantified by counting the number of branch points (C). In vitro tube formation assay (A and B) and tube branch points (C). Representative images are displayed and data are shown as mean ± S.D. (error bars) of counted tube branch points from 4 microscopic fields of three independent replicates. ***p < 0.005. Scale bars, 500 μm.