Table 2. Comparison of genome engineering achieved with homologous recombination (HR) and non-homologous end-joining (NHEJ).

Genome engineering		Screening method	Operational complexity	Large fragment deletion	Genome-scale engineering	References
Principle	Method
NHEJ	CA-NHEJ	Not required	Easy	Available	Efficient	This study
HR	CRISPR-Cas9 assisted HR	Not required	Strenuous	Available	Difficult	10, 29, 43
HR	Double-stranded DNA recombineering	Required	Strenuous	Time consuming	Difficult	8, 9
HR	Single-stranded DNA recombineering	Not required	Easy	Incapable	Efficient	32, 33