Figure 4. Expression of presynaptic proteins is reduced in SPL^fl/fl/Nes mice.

(a–f) Protein amounts were assessed by immunoblotting (unpaired t-test, P_Bassoon = 0.0002, P_Synapsin-1 < 0.0001, P_{syntaxin 1} = 0.0157, P_VAMP2 = 0.0048, P_{Synaptophysin} = 0.0099), and (g) transcript amounts were determined by qRT-PCR in hippocampi of 6-month-old mice. Immunostaining of the presynaptic marker proteins (h) Bassoon and (i) synaptophysin in cerebellar granule cells after 2 weeks in culture (unpaired t-test, P_Bassoon < 0.0001, P_{Synaptophysin} = 0.0034). F-actin (red), bassoon and synaptophysin (green).