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. 2016 Nov 24;6:37643. doi: 10.1038/srep37643

Figure 6. Immunofluorescence analysis of NDK intracellular accumulation.

Figure 6

(A) P. gingivalis-infected primary GECs after treatment with probenecid, MβCD, ML9 or cytochalasin D. Uninfected, untreated cells were used as a control; (B) PNX1 siRNA-transfected P. gingivalis-infected primary GECs with or without MβCD, ML9 or cytochalasin D treatment. Non-target siRNA-transfected P. gingivalis-infected GECs were used as a control. P. gingivalis-NDK is labelled in green, detected by monoclonal P. gingivalis-NDK specific antibody, visualized with anti-rabbit AlexaFluor488 secondary antibody. Bar represents 1 μm. (C) NIH ImageJ analysis was performed for measuring cell fluorescent intensity. Cell boundaries were determined by actin labelling with phalloidin-TRITC. Corrected total cell fluorescence was calculated and measurements were normalized to the mean intensity of the uninfected, untreated cells. All data represent an average of at least three separate experiments. ** Denote P-values < 0.001. Select exact P-values are also shown.