Figure 1. Displaying of folic acid molecules at the tip of a tHBcAg dimer using the nanoglue.

A pentadecapeptide containing the nanoglue, linker and 3 Lys residues interacts with a tHBcAg dimer. The nanoglue binds specifically at the tip of the tHBcAg dimer and its Lys is cross-linked to Asp or Glu using EDC and Sulfo-NHS. Folic acid (FA) molecules are then conjugated to the free Lys residues at the N-terminus of the pentadecapeptide. The tHBcAg dimer self-assembles into nanoparticles.