Figure 2. Mint3 deficiency does not affect the induction of type-I interferons and IFV-specific antibody production.

(a) Viral copy numbers in the infected lungs. The lungs of WT and Mint3^−/− mice (n = 6 per group) at day 0, 4, and 8 following infection with IFV were homogenized. Total RNA was extracted from the infected lungs and viral genome RNA copies were quantified by qPCR. (b) IFN-α and IFN-γ levels in the BALF from WT and Mint3^−/− mice (n = 6 per group) after IFV infection. Data are presented as the means ± SD and are representative of three independent experiments. *P < 0.05, **P < 0.01 by the Student’s t-test. (c) IFV-specific antibody production. WT and Mint3^−/− mice (n = 6 per group) were intranasally infected twice with 10³ PFU of IFV on day 0 and 21. IFV-specific IgG in serum and IgA in the BALF were analysed on day 0 and 7 after the second infection. Data are presented as the means ± SD of triplicates. Data are representative of two independent experiments. *P < 0.05, **P < 0.01 by the Student’s t-test.