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. 2016 Nov 24;6:37885. doi: 10.1038/srep37885

Figure 2. TRIP-1 is present in the ECM of preosteoblasts.

Figure 2

(2a) Expression of TRIP-1 in the secretome of primary calvarial osteoblasts. (2b) Representative confocal micrographs of ECM isolated from MC3T3-E1 preosteoblasts immunostained for TRIP-1. Fibronectin was used as a positive control. The corresponding secondary antibodies were used as negative controls (2c) Representative unstained TEM images of dentin wafers showing the presence of immunogold labeled TRIP-1 (black arrows) in the dentin matrix around the dentinal tubules. (d,e): TRIP-1 is transported to ECM via exosomes. (2d) Western blot analysis of exosomes isolated from MC3T3-E1 cells show the presence of CD-63 an exosome marker and TRIP-1. (2e) Representative TEM image of solubilized exosomes showing the presence of immunogold labeled CD-63 (Black arrows, 20 nm) and TRIP-1 (White arrows, 10 nm). Inset shows the higher magnification image of the boxed area. Gold labeled anti-mouse (10 nm) and anti-rabbit (20 nm) secondary antibodies were used as control.