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. Author manuscript; available in PMC: 2016 Nov 24.
Published in final edited form as: J Neurosurg. 2015 Oct 16;124(6):1562–1567. doi: 10.3171/2015.4.JNS15191

Fig. 3.

Fig. 3

DNA sequencing of tumor IDH1. Sanger sequencing of DNA extracted from the pituitary adenoma revealed a c.394C > T (R132C) mutation of IDH1 (center). Repeat sequencing was performed on DNA derived from remaining paraffin-embedded tissue, using PNA and nested PCR technique, which confirmed previous results (lower). Using the same methodology of PNA and nested PCR, the same c.394C > T mutation was found in DNA from the chondrosarcoma tissue (upper). Figure is available in color online only.